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技術(shù)規(guī)格
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Background: |
Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag was a nine amino acid peptide with high specificity and affinity towards streptavidin which allows the simple purification of protein by use of affinity columns, but required addition to only the C-terminus of recombinant proteins. To also allow a Strep-tag to be placed at the N-terminus of recombinant proteins, it was re-engineered and re-named Strep-tag II. |
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Applications: |
WB |
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Name of antibody: |
Strep tag II |
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Immunogen: |
Synthetic peptide: Strep tag II conjugated to KLH |
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Full name: |
Strep tag II |
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WB Recommended dilution: |
5000-20000 |
