技術(shù)規(guī)格
Background: |
This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. [provided by RefSeq]. |
Applications: |
WB, IHC, IF, FC |
Name of antibody: |
POLR2E |
Immunogen: |
Fusion protein of human POLR2E |
Full name: |
polymerase (RNA) II (DNA directed) polypeptide E, 25kDa (POLR2E) |
Synonyms: |
hRPB25; hsRPB5; RPABC1; RPB5; XAP4 |
SwissProt: |
P19388 |
IHC positive control: |
human breast tissue and carcinoma of human lung tissue; human pancreas tissue and carcinoma of human prostate tissue |
IHC Recommend dilution: |
30-150 |
WB Predicted band size: |
25 kDa |
WB Positive control: |
HepG2, Hela, SVT2, A549, COS7, Jurkat, MDCK, PC12, MCF-7 cell lysates |
WB Recommended dilution: |
500-2000 |